Anders Lee and Ross Johnston were placed in NHL COVID-19 protocol by the Islanders on Friday.
Lee, a forward, played in New York's last game, a 6-1 loss at the Florida Panthers on Tuesday. He has scored four goals in 12 games this season.
Johnston, a forward, hasn't played since a 6-3 opening-night loss at the Carolina Hurricanes on Oct. 14.
They join forward Josh Bailey, who did not play Tuesday, in protocol.
"We found out the news last night, so they are in protocol," Islanders general manager Lou Lamoriello said Friday. "There's no question you don't replace the players that are in protocol, but this is a tremendous group of individuals as far as handling any type of adversity that comes their way."
New York hosts the Calgary Flames on Saturday (7 p.m. ET; SNW, MSG+, MSG+2, ESPN+, NHL LIVE) in the first game at UBS Arena.
"Right now, there's a total preparation for getting ready for tomorrow night," Lamoriello said. "We had a good skate today, good meetings and we're just going along on a normal process. There's nothing we can control."