Correction to: Scientific Reports https://doi.org/10.1038/s41598-021-90219-1, published online 27 May 2021
The original version of this Article contained errors.
In Figure 5 the labels at the top and bottom of the figure were incorrectly captured. The original Figure 5 and accompanying legend appear below.
High-content confocal microscopy autophagy analysis. (a) Left panel illustrates the principle of high content confocal microscopy analysis. Briefly, 1 × 104 pCMV-RFPLC3GFP transfected cells were treated with doxorubicin (500 nM—24 h) and Carmofur (10 µM—24 h). After treatments, cells were fixed and analyzed for GFP + RFP + overlapping puncta and for GFP − /RFP + vesicle content. Around 103 transfected cells/well were analyzed using Harmony algorithms, where RFP + /GFP + vesicles are counted as autophagosomes and RFP + /GFP − vesicles are counted as autolysosomes. The outcome of this test is the following: an autophagy inducer will increase RFP + /GFP + and RFP + /GFP − vesicles, an autophagy blocker will increase RFP + /GFP + but decrease RFP + /GFP − vesicles, whereas an autophagy inhibitor will decrease RFP + /GFP + and RFP + /GFP − vesicles. Right panel shows an overview of a single High-Content acquisition, in which every big square comprises hundreds of 3% overlapping images taken at × 63 magnification. (b) Images taken from the acquisition shown in (a). (c,d) Statistical analysis of RFP + /GFP + and RFP + /GFP − vesicles revealed that A375 cells increases the RFP + /GFP + and RFP + /GFP − vesicles when exposed to doxorubicin, compared to A375 AC-null cells, in which autophagy inhibition was detected. Statistical analyses were performed using one-way ANOVA test (*p < 0.05, **p < 0.01, ***p < 0.001). Data are expressed as mean ± SD.
In addition, the Author Contributions section was incomplete.
“Conceptualization, M.L. and R.A.; methodology, V.L.R.; validation and statistical analysis, R.A; lipidomic assays M.B; revision of manuscript, G.F., D.P. and MP; writing—original draft preparation, V.L.R; writing review and editing, M.L., R.A.; Confocal screenings: M.L., P.S. All authors have read and agreed to the published version of the manuscript.”
now reads:
“Conceptualization, M.L. and R.A.; methodology, V.L.R.; validation and statistical analysis, R.A; lipidomic assays M.B; data curation, funds management PQ; revision of manuscript, G.F., D.P. and MP; writing—original draft preparation, V.L.R; writing review and editing, M.L., R.A.; Confocal screenings: ML, P.S. All authors have read and agreed to the published version of the manuscript.”
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Lai, M., Amato, R., La Rocca, V. et al. Publisher Correction: Acid ceramidase controls apoptosis and increases autophagy in human melanoma cells treated with doxorubicin. Sci Rep 11, 20330 (2021). https://doi.org/10.1038/s41598-021-99797-6
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DOI: https://doi.org/10.1038/s41598-021-99797-6
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